过氧化氢特异性荧光探针BES-H2O2-Ac / BES-H 2O2
• 比二氯荧光黄 (DCFH) 有更高的特异性
• 可动态监测活细胞内的过氧化氢
• BES-H2O2-Ac 可穿透细胞膜，无需打孔
• 极易溶于水，可制成水溶液使用
• 可用于荧光显微镜、流式细胞仪
BES-So-AM：使用举例
图1）是Jurkat T细胞在含33μM BES-So-AM的培养基中37°C培养1小时，使探针进入细胞，然后添加4mM丁酸（O2•-诱导剂），37°C培养1小时；图2）是Jurkat T细胞在含33μM BES-So-AM的培养基中37°C培养1小时，使探针进入细胞，然后不添加丁酸，37°C再培养1小时；图3）是Jurkat T细胞在含33μM BES-So-AM的培养基中37°C培养1小时，然后添加5mM的丁酸，继续培养一个小时。参考文献
1) Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 239 (2004).
2) Maeda, H. et al.: Chem. Pharm. Bull., 49, 294 (2001).
1. Features
•Much higher selectivity compared to conventionally-used DCFH probes•Responds specifically to hydrogen peroxide, enabling detection of intracellular hydrogen peroxide activity•BES-H2O2-Ac is cell membrane permeable•Superior water solubility allows use with aqueous solutions•Can be used in flow cytometry
2. Application
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H2O2-produced stimulation |
No H2O2-produced stimulation |
| Fluorescent images |
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| Phase-contrast images |
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Fluorescent images of Jurkat T cells with BES- H2O2 -Ac and the same-fi eld phase-contrast images
Jurkat T cells were cultured in a medium with 50μM BES-H2O2-Ac for 1 hour. Then, one group of them was cultured in a medium with 5 mM butyric acid (H2O2-produced stimulation), whereas the other in a medium without butyric acid (No H2O2– produced stimulation), each for 1 hour. (Courtesy: Professor Hatsuo Maeda, PhD., School of Pharmacy, Hyogo Univ. of Health Sciences) |
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3. References
- “Fluorescent probe for hydrogen peroxide based on a non-oxidative mechanism”, Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 2389-91 (2004).
- “Hydrogen peroxide-induced deacetylation of acetyl resorufin as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase”, Maeda, H. et al.: Chem. Pharm. Bull., 49, 294-8 (2001).
4. Products List
< All products are for Cellbiology; Keep at room temperature >
|
Cell Permeability |
Product Name |
Package Size |
Wako Catalog No. |
Thiol/Selenol
selective probe |
x
|
BES-Thio |
1 mg
|
025-15481
|
| H2O2 Probes |
o
<Detection of
cell-derived H2O2
|
BES-H2O2-Ac |
1 mg
|
028-17811
|
|
x
|
BES-H2O2 (Cell-impermeant) |
1 mg
|
021-16201
|
Superoxide
selective probe |
o
<Detection of
cell-derived superoxide>
|
BES-So-AM |
1 mg
|
021-17801
|
|
x
|
BES-So (Cell-impermeant) |
1 mg
|
028-16211
|
|
说明书下载： |
http://www.wako-chem.co.jp/english/labchem/product/life/BES-H2O2/pdf/WPUb1_p15-7.pdf
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