The LabAssay™ Series is a collection of biochemical assay kits for analyzing samples from humans, mice, and other animals. Since analyses are performed in microtiter plates, many samples can be concurrently evaluated, and only small sample amounts are needed.
※For Research Use Only, Not for Diagnostic Use

• 1. LabAssay™ Ammonia
• 2. LabAssay™ ALP
• 3. LabAssay™ Cholesterol
• 4. LabAssay™ Creatineine
• 5. LabAssay™ Glucose
• 6. LabAssay™ NEFA
• 7. LabAssay™ Phospholipid
• 8. LabAssay™ Tryglyceride
• 9. Reagents for Adiposity & Diabetes Research

• LabAssay™ Series (1,695KB/6p)

1. LabAssay™ Ammonia

Ammonia is mainly produced in the intestine or the kidney. Ammonia produced in the body is converted to urea through a series of reactions known as the urea cycle in the liver and eliminated through urine.

Assay Principle (Modified Fujii-Okuda Method)

Ammonia is converted to Dioxydiphenylamine by the reaction of Phenol and Sodium Pentacyanonitrosyl ferrate(III). A reaction of the Dioxydiphenylamine with Sodium Hypochlorite produces Indophenol ,which pigments blue, under alkaline conditions. LabAssay ™ Ammonia is a kit used for the quantitative determination of ammonia nitrogen in samples by measuring absorbance of the blue color.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Deproteinizing 100mL×1vial 2 Chromogen Reagent A 50mL×1vial 3 Chromogen Reagent B 25mL×1vial 4 Chromogen Reagent C 50mL×1vial 5 Ammonia Standard Solution 15mL×1vial 6 Dilute olution for Standard 20mL×1vial

Performance

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※Not available for sale in India.

2. LabAssay™ ALP

Alkaline Phosphatase (ALP) is an enzyme distributed in a variety of tissues such as the liver, bone and small intestines in animals. Especially, it is used one of the Osteogenesis Markers in bone metabolism research area.

Assay Principle (Alkaline Phosphatase activity assay with p-Nitrophenyl Phosphate as a substrate)

p-Nitrophenylphosphate is hydrolyzed into p-Nitrophenol in the presence of Alkaline Phosphatase(ALP). LabAssay™ ALP is a kit designed to determine Alkaline Phosphatase activity in samples by measuring the amount of p-Nitrophenol released by p-Nitrophenylphosphate as a phosphatase substrate.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Substrate Tablet 20 tablets 2 Buffer Solution 100mL×1vial 3 Stop Solution 100mL×1vial 4 Standard Solution 10mL×1vial

Performance

Reference

1) Ito, S. et al.: J. Pharmacol. Exp. Ther., 333, 341 (2010).　※Extraction liquid of mouse kidney
2) Iwakura, T. et al.: J. Orthop. Res., 27, 208 (2009).　※Extraction liquid of human culture cells
3) Furuya, Y. et al.: J. Biol. Chem., 286, 37023 (2011).　※Mouse serum
4) Itoh, T. et al.: J. Biol. Chem., 284, 19272 (2009).　※Extraction liquid of mouse culture cells

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3. LabAssay™ Cholesterol

Cholesterol, a major component of cell membranes and the starting material in steroid synthesis in many animals, is a factor behind arteriosclerosis and other vascular diseases.

Assay Principle (Cholesterol Oxidase・DAOS method)

Hydrogen peroxide produced by a reaction of cholesterol and cholesterol oxidase let N-Ethyl-N-（2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt（DAOS）and 4-Aminoantipyrin oxidize and condensate. LabAssay™ Cholesterol is a kit to determine total cholesterol by measuring absorbance of the blue color which is generated by the oxidative condensation reaction.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Buffer 150mL×2vials 2 Chromogen for 150mL×2vials 3 Standard Solution 10mL×1vial

Performance

Reference

1) Wu, S. Y. et al.: Acta Pharmacol. Sin., 30, 202 (2009).　※Mouse plasma
2) Uchida, K. et al.: Exp. Anim., 58, 181 (2009).　※Extraction liquid of mouse tissue
3) Ushio, M. et al.: Am. J. Physiol. Endocrinol. Metab., 305, E293 (2013).　※Extraction liquid of mouse tissue
4) Kobayashi, Y. et al.: Biosci. Biotechnol. Biochem., 74, 2385 (2010). ※ Extraction liquid of rat tissue

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※Not available for sale i n the US.

4. LabAssay™ Creatineline

Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration.

Assay Principle (Jaffé  method)

LabAssay™ Creatinine can be used to measure the creatinin levels in samples by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Depoteinizin Reagent 150mL×2vials 2 Picric Acid Reagent for 150mL×2vials 3 0.75mol/L Sodium Hydroxide Solution 10mL×1vial 4 Standard Solution 10mL×1vial

Performance

Reference

1) Aranami, F. et al.: J. Med. Invest., 57, 95 (2010).　※ Mouse plasma and urine
2) Toyama, K. et al.: Br. J. Pharmacol., 166, 1183 (2012). 　※Mouse plasma
3) Matsumoto, A. et al.: Environ. Health Prev. Med., 15, 211 (2010).　※Human urine
4) Nasrin, S. et al.: J. Pharmacol. Sci., 122, 270 (2013).　※Rat urine

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※Not available for sale in Europe.

5. LabAssay™ Glucose

Sugar is one of the most important sources of energy in biology. It is regulated by various factors within an organism. Glucose converges to a stable ratio of α-form and β-form in solutions.

Assay Principle (Mutarotase・GOD method)

α-D-Glucose is converted to β-D-Glucose by mutarotase. Hydrogen peroxide, which is produced by a reaction between β-D-Glucose and glucose oxidase(GOD), promotes oxidative condensation of phenol with 4-aminoantipyrine quantitatively. LabAssay™ Glucose is a kit used for the quantitative determination of glucose concentrations in samples by measuring absorbance of a red color which is generated by the oxidative condensation reaction.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Buffer 150mL×2vials 2 Chromogen Reagent for 150mL×2vials 3 Glucose Standard I 10mL×1vial 4 Glucose Standard II 10mL×1vial

Performance

Reference

1) Yamashita, Y. et al.: Biosci. Biotechnol. Biochem., 77, 888 (2013).　※Mouse plasma
2) Yamashita, Y. et al.: J. Nutr. Sci., 1, e2 (2012).　※Mouse plasma
3) Wang, W. et al.: J. Renin Angiotensin Aldosterone Syst., 15, 384 (2014).　※Mouse serum
4) Maesako, M. et al.: Neurobiol. Aging, 33, 1011 (2012).　※Mouse serum

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6. LabAssay™ NEFA

NEFA (Non‐esterified fatty acid) in the blood is transported complexed with an albumin to peripheral tissues. They are important sources of fuel for the peripheral tissues. The concentration of NEFA in the blood is regulated by a release from the adipose tissues, a consumption in the periphral tissues or a take up from the liver.

Assay Principle (ACS・ACOD method)

NEFA (Non‐esterified fatty acid) forms Acyl-CoA in the presence of Acyl-CoA synthetase(ACS). Hydrogen peroxide, which is produced by a reaction between the Acyl-CoA and Acyl-CoA oxidase(ACOD), promotes oxidative condensation of 3-methyl-N-ethyl-N（-β-hydroxyethyl）-aniline（MEHA） with 4-aminoantipyrine. LabAssay™ NEFA can be used for the quantitative determination of NEFA in samples by measuring absorbance of a blue purple color which is generated by the oxidative condensation reaction.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Chromogen Reagent A for 10mL×6vials 2 Solvent A 65mL×1vial 3 Chromogen Reagent A for 20mL×6vials 4 Solvent B 10mL×1vial 5 NEFA Standard Solution （Oleic acid 1mEq/L） 10mL×1vial ※Oleic acid 1mEq=1mmol

Performance

Reference

1) Wang, F. et al.: J. Mol. Endocrinol., 52, 133 (2014).　※Mouse plasma
2) Kobayashi, Y. et al.: Biosci. Biotechnol. Biochem., 74, 2385 (2010).　※Rat plasma
3) Chang, Y. C. et al.: EMBO Mol. Med., 5, 1165 (2013).　※Mouse plasma
4) Uchida, K. et al.: Exp. Anim., 58, 181 (2009).　※Mouse serum

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※Not available for sale in the US and Europe.

7. LabAssay™ Phospholipid

Phospholipids are known as not only as major component of cell membranes but also perform vital functions within the body such as emulsification and absorption of fats or coagulation of blood.

Assay Principle (Choline Oxidase・DAOS method)

Phospholipids are hydrolyzed by Phospholipase D to release hydrogen peroxide. The formed hydrogen peroxide promotes oxidative condensation of N-ethyl-N-（2-hydroxy-3-sulfopropyl）-3,5-dimethoxyaniline sodium salt（DAOS) with 4-aminoantipyrine. LabAssay™ Phospholipid is a kit to determine Phospholipid concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Buffer 50mL×8vials 2 Chromogen Substrate for 50mL×8vials 3 Standard Solution 10mL×2vials

Performance

Reference

1) Xu, Q. et al.: Biosci. Biotechnol, Biochem., 77, 1390 (2013).　※Extraction liquid of mouse liver
2) Kuge, H. et al.: J. Biol. Chem., 289, 26783 (2014).　※Liposome
3) Kessler, E. C. et al.: J. Dairy Sci., 97, 5481 (2014).　※Bovine
4) Cheng, L. et al.: Transplantation, 90, 127 (2010).　※Rat

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※Not available for sale in the US.

8. LabAssay™ Tryglyceride

Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides, cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.

Assay Principle

Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase(GPO), promotes oxidative condensation of N-ethyl-N-（2-hydroxy-3-sulfopropyl）-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay ™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Kit Contents　　　　　　　　　　　　　　　　　　　　　Standard Curve

1 Buffer 105mL×3vials 2 Chromogen Substrate for 105mL×3vials 3 Standard Solution 10mL×1vial

Performance

Reference

1) Saito, M. et al.: Biosci. Biotechnol. Biochem., 76, 2049 (2012).　※Mouse serum
2) Fujimori, K. et al.: Prostaglandins Other lipid Mediat., 94, 96 (2011).　※Extraction liquid of mouse culture cells
3) Zhou, L. et al.: Am. J. Physiol. Renal Physiol., 305, F1491 (2013).　※Rat plasma
4) Wu, S. Y. et al.: Acta Pharmacol. Sin., 30, 202 (2009).　※Rat plasma
5) Wang, F. et al.: J. Mol. Endocrinol., 52, 133 (2014).　※Rat plasma
6) Kato, H. et al.: J. Hepatol., 60, 1032 (2014).　※Extraction liquid of mouse liver

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9. Reagents for Adiposity & Diabetes Research

Wako offers a wide variety of products for studying Obesity and Diabetes.

ELISA for Measuring Insulin

Other products : Reagent for Adiposity & Diabetes Research