1,4-b-D-半乳糖内切酶[芽孢杆菌] endo-1,4-β-Galactanase (Cellvibrio japonicus) 货号:E-GALCJ Megazyme中文站

1,4-b-D-半乳糖内切酶[芽孢杆菌]

英文名:endo-1,4-β-Galactanase (Cellvibrio japonicus)

货号:E-GALCJ

规格:1500 Units

High purity recombinant endo-1,4-beta-Galactanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.89 
CAZy Family: GH53

Recombinant. Native full-length Gal53 from Cellvibrio japonicus. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 100 U/mg (40oC, pH 8.0 on potato galactan).

Stability: > 2 years at 4oC.

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美国Clear H2O甜味剂果冻Sucralose

美国Clear H2O甜味剂果冻Sucralose

品牌: 美国Clear H2O
货号: MediGel Sucralose
供应商: 上海金畔生物科技有限公司
保质期: 18个月
保存条件: 常温室温
规格: 96个/箱

Products fo

上海金畔生物科技有限公司是美国Clear H2O公司的中国地区唯一代理商,请放心购买!

MediGel® Sucralose

MediGel Sucralose is a Barrier Packed non-wetting sucralose flavored, low calorie water gel designed to administer medication to your research animal. The sweetener masks medication taste, like a spoon full of sugar!

低热量的甜味剂,掩盖药物本身的味道

Available In:
74-02-5022 2 oz cup (96 Units/Case)

 

4-甲基β-纤维四糖 4-Methylumbelliferyl-β-cellotetraoside 货号:O-4MUG4 Megazyme中文站

4-甲基β-纤维四糖

英文名:4-Methylumbelliferyl-β-cellotetraoside

货号:O-4MUG4

规格:15 mg

CAS: 84325-19-9
Molecular Formula: C34H48O23
Molecular Weight: 824.7
Purity: > 95%

A substrate for research into cellulase (endo-1,4-β-glucanase) or cellulose degrading enzymes.

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2.0ml深孔板 圆孔 美国AXYGEN P-DW-20-C板、AM-2ML-RD盖

名称:2.0ml深孔板 圆孔

品牌:美国AXYGEN

订货号:P-DW-20-C板、AM-2ML-RD盖

2.0ml深孔板  圆孔                                                        美国AXYGEN                                                        P-DW-20-C板、AM-2ML-RD盖

咨询此产品

产品介绍

货号:P-DW-20-C板  AM-2ML-RD盖

厂商:AXYGEN

单位:板:5块/10包/箱

          盖:10块/5包/箱

 原产号

 产品名称

 包装 

单位 

 P-DW-20-C

 圆孔2.0ml深孔板

 5块/包,10包/箱

 箱

 AM-2ML-RD

圆孔深孔板盖

 10块/包,5包/箱

 箱

液体泄漏对策产品的Cicaクリアス皮尔巴拉套件/ピグマット|实验补助剂|一般分析纯试剂|试剂|关东化学株式会社

Cicaクリアス皮尔巴拉套件/ピグマット紧急时的药液泄漏吸收马特对策。马特是吸收能力优秀酸、碱、有机溶剂等1包约18 L的药液吸收可能。吸收剂透明盒子进入。所以残量一眼确认,紧凑的设计是所有的地方可放置。

试剂的咨询

信息查询

电话: 03 – 6214 – 1090

传真: 03 – 3241 – 1047

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甘油检测试剂盒 Glycerol Assay Kit 货号:K-GCROL Megazyme中文站

甘油检测试剂盒

英文名:Glycerol Assay Kit

货号:K-GCROL

规格:70 assays (manual) / 700 assays (microplate)

分析物意义:常见食品组分,或作为甜味剂,或用于改善口感

Megazyme检测试剂盒优点:新型的药片模式,性质更稳定,反应快

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+

Kit size: 70 assays (manual) / 700 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK

Advantages

  • Novel tablet format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual and microplate formats

 

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Is K-GCROL specific for glycerol?

K-GCROL is highly specific for glycerol.
Some compounds that are known not to react or interfere with the assay include:
Polyethylene glycol
Ethylene glycol  
Propylene glycol 

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q5. Is the Glycerol Assay Kit (K-GCROL) suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit.  It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed, by dilution (if required) in distilled water. 

Q6. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q7. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q9. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q10. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q11. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q12. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q13. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q14. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q15. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q16. To measure fermentation samples contain high microbial cell density, is cell disruption required?

Cell disruption is only require to measure glycerol within microbial cells.

To measure glycerol in the extracellular media only then cell disruption is not required and centrifugation of the sample may be sufficient, e.g.:

(a) Determination of glycerol in cell culture media/supernatants. In general, the concentration of glycerol in cell culture media/supernatants can be determined without any sample treatment (except clarification by centrifugation/filtering or dilution according to the dilution table, if necessary). Typically, no clarification or dilution is required, and a sample volume of 0.1 mL is satisfactory.

If interference is suspected then sample clarification/deproteinisation using carrez reagents or perchloric acid should be used (methods are provided in the kit booklet).

1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌] β-D-Xylosidase (Selenomonas ruminantium) 货号:E-BXSR-1KU Megazyme中文站

1,4-b-D-葡聚糖纤维二糖水解酶[单胞菌]

英文名:β-D-Xylosidase (Selenomonas ruminantium)

货号:E-BXSR-1KU

规格:1000 units

EC 3.2.1.37
CAZY Family: GH43

1,4-β-D-xylan xylohydrolase. Recombinant from S. ruminantium. In 3.2 M ammonium sulphate.
Specific activity: ~ 115 U/mg (40oC, pH 5.3 on p-NP-β-D-xylanopyranoside).
Other activities: p-NP-α-L-arabinofuranoside ~ 7 %.

 

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医药化妆品原料|医药/化妆品|关东化学株式会社

关于原料生产销售。

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日本药典(制造专用)医药品

医药化妆品原料|医药/化妆品|关东化学株式会社

产品开发从制造到果实能使用的“制造”专用医药品销售。

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医薬品添加剂规格(JPE),美国药典(USP – NF),欧洲药典(EP)对应了的高纯度溶剂准备着。

原料药合成溶剂

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水分,不挥发物及有害性高的苯为规格保证的高纯度的溶剂产品为您准备了。

日本药典明胶

医药化妆品原料|医药/化妆品|关东化学株式会社

各种各样的特性,拥有高纯度的日局精制明胶备有各种产品。

医药部外品原料

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医药部外品原料规格(外原规)的适合各种化合物)准备了。

医药/化妆品的咨询

信息查询

电话: 03 – 6214 – 1093

传真: 03 – 3241 – 1054

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