确认材料的耐药性 >> 耐药性检索
,
250ml PFA窄口洗瓶日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:3/4、外径×内径(φT):19.05×15.8、L1:150)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
PFA窄口洗瓶(容量100ml)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
高品质无金属窄口洗瓶。
特点:丙酮等有机溶剂、乙醇等醇类、强酸强碱、纯水等都可使用 瓶身、瓶盖、喷嘴全部由PFA制成,所有PFA制的产品都无须担心金属溶出问题,适合微量分析领域实验使用。
可熔性聚四氟乙烯(PFA)弧形管(称呼:1/2、外径×内径(φT):12.7×9.5、L1:500)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
.
2000ml PFA液体运输容器(接口数:2个、适合管规格(外径):3/8inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:1/2、外径×内径(φT):12.7×9.5、L1:400)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2200ml PFA液体运输容器(接口数:3个、适合管规格(外径):1/4inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
KAPA Biosystems HiFi高保真酶 KK2601 KK2602 中国代理商
KAPA Biosystems2016 报价表 KAPA Biosystems试剂官网代理商
KAPA Biosystems HiFi高保真酶 KK2601 KK2602 中国代理商
KAPA BIOSYSTEMS2016 报价表
KAPA Biosystems是一家美国知名生物公司,成立于2006年,其生产基地位于南非开普敦。KAPABiosystems致力于研发、生产下一代PCR(Next-Generation PCR)所需的生物酶。目前其分子进化平台开发的产品已被广泛应用于DNA扩增、二代测序、分子诊断等领域。
目前大部分生物化学实验、科学研究主要应用较初级的生物酶,因受到这些酶本身特性的限制而无法更好地开展工作。近年来酶分子进化技术的出现,可专为某些特定的实验量身定制特定的生物酶。分子进化技术(酶工程)打破了现有的研究局限,为生物研究开创了一片新天地。
KAPA Biosystems技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。
KAPA Biosystems公司追求科学研究与市场化运作的完美结合,执着于“科技创造完美产品,助力生命科学研究”这一zui高宗旨。
KAPA Biosystems产品价格 | ||||
产品归类 | Product Code | Product Category | Quantity | 目前目录售价 |
HiFi高保真酶 | KK2101 | HiFi | 100 units | 700 |
KK2102 | HiFi | 250 units | 1600 | |
KK2501 | HiFi Hot Start | 100 units | 800 | |
KK2502 | HiFi Hot Start | 250 units | 1800 | |
KK2601 | HiFi Hot Start | 100 rxn | 800 | |
KK2602 | HiFi Hot Start | 500 rxn | 3500 | |
HiFi高保真酶(二代测序专用) | KK2611 | HiFi Hot Start for NGS | 50 rxn | 1100 |
KK2612 | HiFi Hot Start for NGS | 250 rxn | 3500 | |
KK2701 | HiFi Hot Start for NGS | 50 rxn | 2400 | |
KK2702 | HiFi Hot Start for NGS | 250 rxn | 10000 | |
KK2801 | KAPA HiFi HS Uracil +ready Mix | 50 x 50µL reactions | 3000 | |
KK2802 | KAPA HiFi HS Uracil +ready Mix | 250 x 50µL reactions | 14000 | |
长片段酶 | KK3006 | Long Range | 100 units | 800 |
KK3005 | Long Range | 250 units | 1600 | |
KK3501 | Long Range Hot Start | 100 units | 900 | |
KK3502 | Long Range Hot Start | 250 units | 1800 | |
KK3503 | Long Range Hot Start | 500 units | 3000 | |
KK3601 | Long Range Hot StartReady Mix with DYE (100 x 25µL rxn) | (100 x 25µL rxn) | 1100 | |
KK3602 | Long Range Hot StartReady Mix with DYE (500 x 25µL rxn) | (500 x 25µL rxn) | 4500 | |
与takara酶相对应的KAPA Extaq酶 | KK3009 | KAPATaq Extra(针对takara ExTaq) | 250units | 400 |
KK3505 | KAPATaq Extra Hot Start(针对takara ExTaq) | 250units | 520 | |
KK3604 | KAPATaq Extra Hot Start Ready Mix with DYE (针对takara ExTaq) | (100 x 25µL rxn) | 400 | |
KK3605 | KAPATaq Extra Hot StartReady Mix with DYE(针对takara ExTaq) | (500 x 25µL rxn) | 1800 | |
KK3606 | KAPATaq Extra Hot Start Ready Mix with DYE | (200 x 25µL rxn) | 800 | |
HRM | KK4201 | KAPA HRM FAST | 1ml | 700 |
KK4202 | KAPA HRM FAST | 5ml | 3200 | |
KK4203 | KAPA HRM FAST | 10ml | 6000 | |
SYBR荧光定量 | KK4600 | SYBR® Fast通用型 | 1 ml | 400 |
KK4601 | SYBR® Fast通用型 | 5 ml | 1800 | |
KK4602 | SYBR® Fast通用型 | 10 ml | 3500 | |
KK4618 | SYBR® Fast通用型 | 50 ml | 17000 | |
KK4603 | SYBR® Fast(ABI Prism) | 1 ml | 400 | |
KK4604 | SYBR® Fast(ABI Prism) | 5 ml | 1800 | |
KK4605 | SYBR® Fast(ABI Prism) | 10 ml | 3500 | |
KK4617 | SYBR® Fast(ABI Prism) | 50ml | 17000 | |
KK4606 | SYBR® Fast(伯乐专用型) | 1 ml | 400 | |
KK4607 | SYBR® Fast(伯乐专用型) | 5 ml | 1800 | |
KK4608 | SYBR® Fast(伯乐专用型) | 10 ml | 3500 | |
KK4609 | SYBR® Fast (Roche LC 480) | 1 ml | 400 | |
KK4610 | SYBR® Fast(Roche LC 480) | 5 ml | 1800 | |
KK4611 | SYBR® Fast(Roche LC 480) | 10 ml | 3500 | |
KK4650 | 1-step qRT-PCR Kit(通用型) | 1ml | 1000 | |
KK4651 | 1-step qRT-PCR Kit(通用型) | 5ml | 4500 | |
KK4652 | 1-step qRT-PCR Kit(通用型) | 10ml | 8000 | |
KK4660 | 1-step qRT-PCR Kit(ABI Prism) | 1ml | 1000 | |
KK4661 | 1-step qRT-PCR Kit(ABI Prism) | 5ml | 4500 | |
KK4662 | 1-step qRT-PCR Kit(ABI Prism) | 10ml | 8000 | |
KK4670 | 1-step qRT-PCR Kit(伯乐专用型) | 1ml | 1000 | |
KK4671 | 1-step qRT-PCR Kit(伯乐专用型) | 5ml | 4500 | |
KK4672 | 1-step qRT-PCR Kit(伯乐专用型) | 10ml | 8000 | |
KK4680 | 1-step qRT-PCR Kit(Roche LC 480) | 1ml | 1000 | |
KK4681 | 1-step qRT-PCR Kit(Roche LC 480) | 5ml | 4500 | |
KK4682 | 1-step qRT-PCR Kit(Roche LC 480) | 10ml | 8000 | |
PROBE荧光定量 | KK4701 | PROBE FAST(通用型) | 1 ml | 400 |
KK4702 | PROBE FAST(通用型) | 5 ml | 1800 | |
KK4703 | PROBE FAST(通用型) | 10 ml | 3500 | |
KK4705 | PROBE FAST(ABI Prism) | 1 ml | 400 | |
KK4706 | PROBE FAST(ABI Prism) | 5 ml | 1800 | |
KK4707 | PROBE FAST(ABI Prism) | 10 ml | 3500 | |
KK4709 | PROBE FAST(伯乐专用型) | 1 ml | 400 | |
KK4710 | PROBE FAST(伯乐专用型) | 5 ml | 1800 | |
KK4711 | PROBE FAST(伯乐专用型) | 10 ml | 3500 | |
KK4715 | PROBE FAST(ABI Prism,定制50ml体系) | 50 | 17000 | |
KK4716 | PROBE FAST(通用型,定制50ml体系) | 50 | 17000 | |
KK4716 | PROBE FAST (ROX Low Mastermix) | 1ml | 400 | |
KK4717 | PROBE FAST(ROX Low Mastermix) | 5 ml | 1800 | |
KK4718 | PROBE FAST(ROX Low Mastermix) | 10 ml | 3500 | |
KK4719 | PROBE FAST(ROX Low Mastermix) | 50 ml | 17000 | |
KK4752 | PROBE FAST(1-step qRT-PCR Probe Universal) | 5 ml | 4500 | |
2G Robust酶及相关产品 | KK5023 | 2G Robust | 100 units | 400 |
KK5024 | 2G Robust | 250 units | 900 | |
KK5004 | 2G Robust(含dNTPs) | 100units | 400 | |
KK5005 | 2G Robust(含dNTPs) | 250 units | 900 | |
kk5522 | 2G Robust Hot Start | 100units | 600 | |
KK5515 | 2G Robust Hot Start | 250 units | 1400 | |
KK5517 | 2G Robust Hot Start | 500 units | 2400 | |
KK5525 | 2G Robust Hot Start | 2500units | 9000 | |
KK5532 | 2G Robust Hot Start(含dNTPs) | 100 units | 700 | |
KK5516 | 2G Robust Hot Start(含dNTPs) | 250 units | 1500 | |
KK5518 | 2G Robust Hot Start(含dNTPs) | 500units | 2800 | |
KK5701 | 2G Robust Hot Start Readymix | 100rxn | 700 | |
KK5702 | 2G Robust Hot Start Readymix | 500 rxn | 2600 | |
2GFast酶及相关产品 | KK5020 | 2G Fast | 100 units | 400 |
KK5021 | 2G Fast | 250 units | 900 | |
KK5008 | 2G Fast(含dNTPs) | 100 units | 500 | |
KK5009 | 2G Fast(含dNTPs) | 250 units | 1100 | |
KK5101 | 2G Fast Ready Mix+ Dye | 100rxn | 400 | |
KK5102 | 2G Fast Ready Mix +Dye | 500 rxn | 1400 | |
KK5523 | 2G Fast Hot Start | 100 units | 600 | |
KK5503 | 2G Fast Hot Start | 250 units | 1300 | |
KK5501 | 2G Fast Hot Start | 500 units | 2400 | |
KK5519 | 2G Fast Hot Start | 2500 units | 7000 | |
KK5530 | 2G Fast Hot Start(含dNTPs) | 100 units | 700 | |
KK5502 | 2G Fast Hot Start(含dNTPs) | 250uinits | 1500 | |
KK5500 | 2G Fast Hot Start(含dNTPs) | 500 units | 2600 | |
KK5513 | 2G Fast Hot Start(Buffer无Mg2+) | 250 units | 1400 | |
KK5511 | 2G Fast Hot Start(Buffer无Mg2+) | 500 units | 2400 | |
kk5512 | 2G Fast Hot Start(含dNTPs,Buffer无Mg2+) | 250 units | 1500 | |
KK5510 | 2G Fast Hot Start(含dNTPs,Buffer无Mg2+) | 500 units | 2800 | |
KK5603 | 2G Fast Hot Start ReadyMix | 100 rxn | 400 | |
KK5601 | 2G Fast Hot Start ReadyMix | 500 rxn | 1400 | |
KK5801 | 2G Fast Hot Start | 100 rxn | 500 | |
KK5802 | 2G Fast Hot Start | 500 rxn | 1900 | |
血液直接扩增 | KK7002 | Blood PCR Kit A | 500 rxn | 2500 |
KK7003 | Blood PCR Kit B | 500 rxn | 2500 | |
KK7004 | Blood PCR Kit A | 1000 rxn | 4500 | |
KK7005 | Blood PCR Kit B | 1000 rxn | 4500 | |
DNA快速提取及扩增 | KK7100 | Express Extract | 50 rxn | 400 |
KK7101 | Express Extract | 100 rxn | 700 | |
KK7102 | Express Extract | 250 rxn | 1500 | |
KK7103 | Express Extract | 500 rxn | 2600 | |
KK7151 | Express Extract+ 2GR HS | 100 rxn combo | 1200 | |
KK7152 | Express Extract+ 2GR HS | 500 rxn combo | 4600 | |
植物直扩 | KK7251 | KAPA3G Plant PCR | 250 units | 3000 |
KK7252 | KAPA3G Plant PCR | 500 units | 5400 | |
Mouse Genotyping相关产品 | KK7301 | Mouse Genotyping Kit | 100 rxn | 800 |
KK7302 | Mouse Genotyping Kit | 500 rxn | 4000 | |
KK7351 | HS Mouse Genotyping Kit | 100 rxn | 1200 | |
KK7352 | HS Mouse Genotyping Kit | 500 rxn | 5000 | |
KK5620 | 2G Fast HotStart Genotyping PCR Kit | 100 rxn | 500 | |
KK5621 | 2G Fast HotStart Genotyping PCR Kit | 500 rxn | 2000 | |
KK8230 | KAPA LTP文库构建(含文库扩增)试剂盒-Illumina | 10个文库 | 2500 | |
KK8231 | KAPA LTP文库构建(不含文库扩增)试剂盒-Illumina | 10个文库 | 2400 | |
KK8232 | KAPA LTP文库构建(含文库扩增)试剂盒-Illumina | 50个文库 | 11000 | |
KK8233 | KAPA LTP文库构建(不含文库扩增)试剂盒-Illumina | 50个文库 | 10500 | |
KK8234 | KAPA HTP文库构建(含文库扩增)试剂盒-Illumina | 96个文库 | 18000 | |
KK8235 | KAPA HTP文库构建(不含文库扩增)试剂盒-Illumina | 96个文库 | 17000 | |
KK8260 | KAPA TruSeq Adapter Kit, (Illumina, 20 μl each) | 144 rxn | 14000 | |
KK2620 | KAPA Library Amplification Kit with Primers (50 x 50 µl reactions) | 50rxn | 1800 | |
KK2621 | KAPA Library Amplification Kit with Primers (250 x 50 µl reactions) | 250rxn | 8000 | |
KK2623 | KAPA Library Amplification Primer Kit (250 x 50 μl rxns) | 250rxn | 1500 | |
KK8400 | KAPA Stranded RNA-Seq Library Preparation Kit (Illumina,24 reactions) | 24 rxn | 12200 | |
KK8401 | KAPA Stranded RNA-Seq Library Preparation Kit (96 reactions) | 96 rxn | 38000 | |
KK8420 | KAPA Stranded mRNA-Seq Library Preparation Kit (24 reactions) | 24 rxn | 14000 | |
KK8421 | KAPA Stranded mRNA-Seq Library Preparation Kit (96 reactions) | 96 rxn | 44000 | |
KK8500 | KAPA Hyper Prep Kit (8 reactions) | 8 rxn | 3100 | |
KK8501 | KAPA Hyper Prep Kit, no amplification (8 reactions) | 8 rxn | 2970 | |
KK8502 | KAPA Hyper Prep Kit (24 reactions) | 24 rxn | 8100 | |
KK8503 | KAPA Hyper Prep Kit, no amplification (24 reactions) | 24 rxn | 7830 | |
KK8504 | KAPA Hyper Prep Kit (96 reactions) | 96 rxn | 28100 | |
KK8505 | KAPA Hyper Prep Kit, no amplification (96 reactions) | 96 rxn | 27000 | |
KK8300 | Ion Torrent Library Preparation Kit (8 reactions) | 8 rxn | 2520 | |
KK8301 | Ion Torrent Library Preparation Kit (48 reactions) | 48 rxn | 13860 | |
KK8310 | Ion Torrent Library Preparation Kit, no amplification (8 reactions) | 8 rxn | 2280 | |
KK8311 | Ion TorrentLibrary Preparation Kit, no amplification (48 reactions) | 48 rxn | 12420 | |
KK8330 | Adapter Kit, non-Barcoded (8 reactions Ion Torrent | 8 rxn | 1200 | |
KK8331 | Adapter Kit, Barcodes 1 – 8 (48 reactions) Ion Torrent | 48 rxn | 7500 | |
KK8332 | Adapter Kit, Barcodes 9 – 16 (48 reactions) Ion Torrent | 48 rxn | 7500 | |
KK8333 | Adapter Kit, Barcodes 17 – 24 (48 reactions) Ion Torrent | 48 rxn | 7500 | |
二代测序文库定量:标准品+引物预混物 | KK4800 | DNA Quant Kit Roche 454 FLX | Each | 6000 |
KK4801 | Primer Premix Roche 454 FLX | Each | 3000 | |
KK4802 | DNA Quant Kit Roche 454 Titanium | Each | 6000 | |
KK4803 | Primer Premix Roche 454 Titanium | Each | 3000 | |
KK4804 | DNA Quant Kit Illumina Genome Analyzer | Each | 6000 | |
KK4805 | Primer Premix Illumina Genome Analyzer | Each | 3000 | |
KK4806 | DNA Quant Kit ABI SOLiD | Each | 6000 | |
KK4807 | Primer Premix ABI SOLiD | Each | 3000 | |
KK4808 | DNA Quant Kit Illumina GA Revised Primers | Each | 6000 | |
KK4809 | Primer Premix Illumina GA Revised Primers | Each | 3000 | |
KK4812 | DNA Quant Kit – Ion Torrent | Each | 6000 | |
KK4813 | DNA Quantification Primer Premix – Ion Torrent | Each | 3000 | |
二代测序文库定量:标准品 | KK4900 | Library Quantification DNA Standards (454 FLX/Lib-A) | 6 x 80 µl | 4000 |
KK4902 | Library Quantification DNA Standards (SOLiD) | 6 x 80 µl | 4000 | |
KK4903 | Library Quantification DNA Standards (Illumina) | 6 x 80 µl | 4000 | |
KK4904 | Library Quantification DNA Standards (Ion Torrent) | 6 x 80 µl | 4000 | |
二代测序zui终文库定量产品 | KK4820 | Library Quant Kit,Roche 454 FLX-SYBR Fast Universal | Each | 7000 |
KK4821 | Library Quant Kit,Roche 454 Titanium-SYBR Fast Universal | Each | 7000 | |
KK4824 | Library Quant Kit,Illumina GA revised primers -SYBR Fast Universal | Each | 7000 | |
KK4823 | Library Quant Kit,ABI SOLiD-SYBR Fast Universal | Each | 7000 | |
KK4830 | Library Quant Kit,Roche 454 FLX-SYBR Fast ABI Prism | Each | 7000 | |
KK4831 | Library Quant Kit,Roche 454 Titanium-SYBR Fast ABI Prism | Each | 7000 | |
KK4835 | Library Quant Kit,Illumina GA revised primers -SYBR Fast ABI Prism | Each | 7000 | |
KK4833 | Library Quant Kit,ABI SOLiD-SYBR Fast ABI Prism | Each | 7000 | |
KK4840 | Library Quant Kit,Roche 454 FLX-SYBR Fast Bio-Rad iCycler | Each | 7000 | |
KK4841 | Library Quant Kit,Roche 454 Titanium-SYBR Fast Bio-Rad iCycler | Each | 7000 | |
KK4844 | Library Quant Kit,Illumina GA revised primers-SYBR Fast Bio-Rad iCycler | Each | 7000 | |
KK4843 | Library Quant Kit,ABI SOLiD-SYBR Fast Bio-Rad iCycler | Each | 7000 | |
KK4850 | Library Quant Kit,Roche 454 FLX-SYBR Fast Roche Light Cycler480 | Each | 7000 | |
KK4851 | Library Quant Kit,Roche 454 Titanium-SYBR Fast Roche Light Cycler 480 | Each | 7000 | |
KK4854 | Library Quant Kit,Illumina GA revised primers-SYBR Fast Roche Light Cycler480 | Each | 7000 | |
KK4853 | Library Quant Kit,ABI SOLiD-SYBR Fast Roche Light Cycler480 | Each | 7000 | |
KK4834 | Library Quant Kit,Illumina GA ABI Prism Custom Kit- Sanger | Each | 7000 | |
KK4827 | Library Quant Kit,Ion Torrent PGM universal | Each | 7000 | |
KK4838 | Library Quant Kit,Ion Torrent PGM ABI Prism | Each | 7000 | |
KK4847 | Library Quant Kit,Ion Torrent PGM Bio-Rad iCycler | Each | 7000 | |
KK4857 | Library Quant Kit,Ion Torrent PGM Roche Light Cycler480 | Each | 7000 | |
人类基因组专用定量Kit(文库构建之前用,主要检测提取的DNA量) | KK4960 | hg DNA Quant & QC kit with SYBR Universal qPCR Mastermix | Each | 8000 |
KK4961 | hg DNA Quant & QC kit with SYBR ABI Prism qPCR Mastermix | Each | 8000 | |
KK4962 | hg DNA Quant & QC kit with SYBR Bio-Rad qPCR Mastermix | Each | 8000 | |
KK4963 | hg DNA Quant & QC kit with SYBR Light Cycler 480 qPCR Mastermix | Each | 8000 | |
KK4964 | hg DNA Quant & QC DNA Standards Kit | Each | 6000 | |
KK4965 | hg DNA Quant & QC and 41 bp Primer Kit | Each | 1500 | |
KK4966 | hg DNA Quant & QC and 129 bp Primer Kit | Each | 1500 | |
KK4967 | hg DNA Quant & QC and 305 bp Primer Kit | Each | 1500 | |
KK4969 | hg DNA Quant & QC Kit with ROX Low qPCR Mastermix | Each | 8000 | |
普通Taq酶及T4连接酶 | ||||
产品归类 | Product Code | Product Category | Quantity | |
Taq相关产品 | KK1014 | Taq 酶 | 250 units | 380 |
KK1015 | Taq 酶 | 500 units | 700 | |
BK1000 | Taq 酶 | 2500 units | 2500 | |
BK1002 | Taq 酶 | 5000 units | 3800 | |
KK1008 | Taq 酶(含dNTPs) | 250 units | 450 | |
KK1016 | Taq 酶(含dNTPs) | 500 units | 800 | |
BK1001 | Taq 酶(含dNTPs) | 2500 units | 3500 | |
BK1003 | Taq 酶(含dNTPs) | 5000 units | 5500 | |
KK1006 | Taq 酶 ReadyMix | 250×50µl反应 | 900 | |
KK1020 | Taq 酶Buffer w/ loading dye | 250 units | 380 | |
KK1022 | Taq 酶Buffer w/ loading dye | 500 units | 700 | |
BK1004 | Taq 酶Buffer w/ loading dye | 2500 units | 2500 | |
BK1006 | Taq 酶Buffer w/ loading dye | 5000 units | 3800 | |
KK1021 | Taq 酶Buffer w/ loading dye(含dNTPs) | 250 units | 450 | |
KK1023 | Taq 酶Buffer w/ loading dye(含dNTPs) | 500 units | 800 | |
BK1005 | Taq 酶Buffer w/ loading dye(含dNTPs) | 2500 units | 3500 | |
BK1007 | Taq 酶Buffer w/ loading dye(含dNTPs) | 5000 units | 5500 | |
KK1024 | Taq 酶ReadyMix w/loading dye | 250×50µl反应 | 900 | |
KK1508 | 热启动Taq酶 | 250 units | 800 | |
KK1510 | 热启动Taq酶 | 500 units | 1200 | |
KK1513 | 热启动Taq酶 | 2500 units | 5000 | |
KK1509 | 热启动Taq酶(含dNTPs) | 250 units | 900 | |
KK1511 | 热启动Taq酶(含dNTPs) | 500 units | 1500 | |
KK1512 | 热启动Taq酶without buffers | 2500 units | 5000 | |
T4DNA连接酶 | KK6010 | T4 DNA Ligase,5units/µL (Weiss Units) | 250 units | 600 |
KK6011 | T4 DNA Ligase,5units/µL (Weiss Units) | 1250 units | 2500 | |
KK6110 | T4 DNA Ligase,Rapid Ligation Kit | 50 rxn | 1400 | |
KK6111 | T4 DNA Ligase,Rapid Ligation Kit | 150 rxn | 3800 |
KAPA KK2611/KK2612 文库扩增试剂盒
KAPA KK2611/KK2612 文库扩增试剂盒
KAPA HiFi HotStart ReadyMix高保真DNA聚合酶预混液
英文名称:KAPA HiFi HotStart ReadyMix
中文名称:KAPA HiFi高保真热启动DNA聚合酶预混液
KAPA HiFi HotStart ReadyMix是一种DNA扩增预混液,包含引物和模板之外扩增完全反应必需成分。适用PCR、NGS高通量测序文库等的扩增。预混液所含KAPA HiFi DNA Polymerase是新型改造B族DNA聚合酶,错误率比野生型Taq DNA聚合酶低约100倍,成功率和产率比野生型B族proofreading活性的DNA聚合酶更高。
HiFi 热启动高保真DNA聚合酶应用
用于常规测序的PCR(直接测序或克隆PCR产物的测序)
新一代测序文库扩增(与KAPA文库扩增方案配合使用时)
扩增片段DNA用于克隆和蛋白质表达或基因组表征
定点突变
KAPA HiFi DNA聚合酶扩增特点
高DNA亲和力,无需辅助蛋白和DNA结合结构域
高持续合成能力
长片段扩增能力
选择失活,低非特异性扩增
KAPA HiFi HotStart DNA聚合酶是一种新型B家族DNA聚合酶,经过工程改造后对DNA的亲和力增加,不需要辅助蛋白或DNA结合域。与野生型B家族DNA聚合酶相比,该酶固有的高持续合成能力可显著提高合成产量,速度和灵敏度。此外,扩增长片段以及富含GC和AT片段的能力显著提高。该酶与特有抗体结合,*次变性步骤之前可以保持失活状态。这可以防止反应过程中的非特异性扩增,提高灵敏度,并提高反应效率。
组份(除引物和模板的扩增反应液)
DNA聚合酶+缓冲液混合
— DNA聚合酶:0.5U /25μL反应
— 缓冲液:dNTPs(每种dNTP 0.3mM,1X),MgCl2(2.5mM,1X )和稳定剂
KAPA HiFi高保真DNA聚合酶技术参数
聚合酶种类:改造B族DNA聚合酶
聚合酶活性:KAPA HiFi HotStart DNA聚合酶具有5’g3’聚合酶和3’g5’核酸外切酶(proofreading)活性,但没有5’g3’核酸外切酶活性。
扩增片段长度:可从基因组DNA中扩增高达15kb的目标或从较不复杂的目标扩增20kb。
KAPA HiFi高保真DNA聚合酶预混液设计用于各种目标和大小片段的常规高保真PCR。它的错误率比野生型Taq DNA聚合酶低约100倍,成功率和产率比野生型B家族(proofreading)DNA聚合酶更高。另外,KAPA HiFi HotStart比野生型B家族DNA聚合酶需要更短的反应时间。 KAPA HiFi HotStart DNA聚合酶具有5’g3’聚合酶和3’g5’核酸外切酶(proofreading)活性,但没有5’g3’核酸外切酶活性。
可熔性聚四氟乙烯(PFA)弧形管(称呼:1/2、外径×内径(φT):12.7×9.5、L1:300)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2200ml PFA液体运输容器(接口数:2个、适合管规格(外径):3/8inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:1/2、外径×内径(φT):12.7×9.5、L1:200)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
,
2200ml PFA液体运输容器(接口数:2个、适合管规格(外径):1/4inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:1/2、外径×内径(φT):12.7×9.5、L1:150)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2000ml PFA液体运输容器(接口数:7个、适合管规格(外径):3/8inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:3/8、外径×内径(φT):9.5×6.3、L1:500)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2000ml PFA液体运输容器(接口数:7个、适合管规格(外径):1/4inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2000ml PFA液体运输容器(接口数:3个、适合管规格(外径):3/8inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:3/8、外径×内径(φT):9.5×6.3、L1:500)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2000ml PFA液体运输容器(接口数:3个、适合管规格(外径):1/4inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:3/8、外径×内径(φT):9.5×6.3、L1:400)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2000ml PFA液体运输容器(接口数:2个、适合管规格(外径):1/2inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
可熔性聚四氟乙烯(PFA)弧形管(称呼:3/8、外径×内径(φT):9.5×6.3、L1:300)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
2000ml PFA液体运输容器(接口数:2个、适合管规格(外径):1/4inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
1000ml PFA液体运输容器(接口数:7个、适合管规格(外径):3/8inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
1000ml PFA液体运输容器(接口数:7个、适合管规格(外径):1/4inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
1000ml PFA液体运输容器(接口数:3个、适合管规格(外径):3/8inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
1000ml PFA液体运输容器(接口数:3个、适合管规格(外径):1/4inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
1000ml PFA液体运输容器(接口数:2个、适合管规格(外径):1/2inch)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索