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4-硝基苯基-β-D-木糖 4-Nitrophenyl-β-D-xylopyranoside 货号：O-PNPX Megazyme中文站

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4-硝基苯基-β-D-木糖

英文名：4-Nitrophenyl-β-D-xylopyranoside

货号：O-PNPX

规格：1 gram

Synonyms: p-Nitrophenyl-β-D-xylopyranoside,
                   pNP-β-D-xylopyranoside
CAS: 2001-96-9
Molecular Formula: C11H13NO7
Molecular Weight: 271.22
Purity: > 98%

High purity 4-Nitrophenyl-β-D-xylopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of β-xylosidase activity.

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1，3-b-D葡聚糖内切酶[木霉菌] endo-1，3-β-D-Glucanase (Trichoderma sp.) 货号：E-LAMSE Megazyme中文站

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1，3-b-D葡聚糖内切酶[木霉菌]

英文名：endo-1，3-β-D-Glucanase (Trichoderma sp.)

货号：E-LAMSE

规格：100 Units

High purity endo-1,3-beta-Glucanase (Trichoderma sp.) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.39

From Trichoderma sp. Highly purified. 
In 3.2 M ammonium sulphate.

Specific Activity: ~ 15 U/mg (40oC, pH 4.5, CM-Curdlan as substrate).

Stable at 4oC for > 4 years.

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转化酶 呋喃果糖苷酶（酵母） Invertase (fructofuranosidase) (yeast) 货号：E-INVRT Megazyme中文站

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转化酶 呋喃果糖苷酶（酵母）

英文名：Invertase (fructofuranosidase) (yeast)

货号：E-INVRT

规格：100 mL; 200000 Units. 2000 U/mL

High purity Invertase (beta-Fructofuranosidase) (liquid) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.26

From yeast. Chromatographically purified. 
In 50% glycerol.

Specific activity: 300 U/mg protein (40oC, pH 4.5, 1% sucrose). 2,000 U/ml. 

Stable at 4oC for > 4 years.

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Amresco 刚果红 573-58-0

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  • 产品名称：提供Amresco的刚果红
  • 产品货号：0379
  • 规格：50G
  • 品牌商标：Amresco
产品名称：Amresco的刚果红

产品说明：A pH indicator (Blue to Red; range 3.0 – 5.0). A cytoplasmic stain for tissues fixed in Bouin’s. Also a specific stain for amyloid.

Grade:CERTIFIABLE GRADE

CAS Number:573-58-0

Synonym(s):None

Molecular Weight:696.67

Molecular Formula:C32H22N6O6S2Na2

Storage Condition:ROOM TEMPERATURE

Absorbance Maximum (nm)    497 – 500

Dye Content (%)    75.0

P-15/P+15    0.99 – 1.04

Alpha葡[萄]糖苷酶[嗜热脂肪芽孢杆菌] α-Glucosidase (Bacillus stearothermophilus) 货号：E-TSAGL Megazyme中文站

发表于由

Alpha葡[萄]糖苷酶[嗜热脂肪芽孢杆菌]

英文名：α-Glucosidase (Bacillus stearothermophilus)

货号：E-TSAGL

规格：1500 Units

High purity alpha-Glucosidase (B. stearothermophilus) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.20 
CAZy Family: GH13

From B. stearothermophilus. 
In 3.2 M ammonium sulphate.

Specific activity: > 80 U/mg (40oC, pH 6.5, p-nitrophenyl α-D-glucoside).

Stable at 4oC for > 4 years.

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Beta葡聚糖[大麦；中黏度] Beta-Glucan (Barley;Med Viscosity) 5g 货号：P-BGBM Megazyme中文站

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Beta葡聚糖[大麦；中黏度]

英文名：Beta-Glucan (Barley;Med Viscosity) 5g

货号：P-BGBM

规格：5 grams

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 1. Which barley beta-glucan do you recommend for the glucanase assay? There are three kinds of barley beta-glucan availabale from Megazyme – high viscosity, medium viscosity and low viscosity.

For glucanase assay, we recommend the medium viscosity beta-glucan.  We now offer low, medium and high viscosity wheat arabinoxylans, and we think that the low viscosity material will be best (easiest) to use in the reducing-sugar assay.

2. Both substrates; barley beta-glucan and wheat arabinoxylan, are standardised to a specific viscosity, e.g. 23-24 cSt. Are the substrates adjusted to give this viscosity?

The substrates are enzymically treated to yield the required viscosity (20 ~ 30 cSt). Modification of the viscosity of beta-glucan is required for IOB viscometric malt beta-glucanase test (which works well).

3. I would like to vacuum dry the standards instead of measuring the moisture content. I wish to dry them at a temperature lower than used in the preparation in Megazyme. Would you please give me some suggestions?

We suggest that you dry the β-glucan powders further in a vacuum oven at about 50˚C.  This will lower the moisture content to approximately 1-2%.

4. I understand a specific polymer can be used for the assay of malt β-glucanase and celluloses. I would very much like to know which polymer this β-glucan is?

The polymer you require is barley beta-glucan (medium viscosity).  The beta-glucan that we supply is pure 1,3:1,4-beta-D-glucan.  Barley β-glucan is also susceptible to hydrolysis by cellulase.

5. Although not specified on the beta-glucan data sheet, is the ratio of 1-3 to 1-4 bonds measured? If so, what is the ratio and would you expect it to remain standardised over a number of different batches?

The content of 1,3 bonds in barley beta-glucan is about 32% (from literature).  We would not expect this to change much (if at all) over different batches.

6. We have measured the molecular weight of beta-glucan originating from barley ordered from your company. Can you please tell us which method have you used for measuring molecular weight?

The MW’s were determined by Multiangle laser light scattering technique.

7. I have purchased barley beta-glucan (lot 30108) and carob galactomannan low viscosity (lot 30702) and would like to know what else there might be in these substrates.

Barley beta-glucan lot 30108 would contain about 3-4% arabinoxylan (this was produced in 1993).  Material supplied post 1995 contains < 0.5% arabinoxylan.  Carob galactomannan is quite pure (> 96%).

8. Low, Medium, High Viscosity β-Glucans, are they naturally occurring at these MW’s or are the MW fractions obtained by some other process?

The high molecular weight beta-glucan is the naturally occurring; this is enzymically modified to give the other viscosities. 

葡萄糖基-麦芽三糖基-麦芽三糖 63-A-D-Glucosyl-Maltotriose – 50mg 货号：O-GMT Megazyme中文站

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葡萄糖基-麦芽三糖基-麦芽三糖

英文名：63-A-D-Glucosyl-Maltotriose – 50mg

货号：O-GMT

规格：50 mg

CAS: 35175-16-7
Molecular Formula: C24H42O21
Molecular Weight: 666.6
Purity: > 95%

High purity 63-alpha-D-Glucosyl-maltotriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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蛋白酶[枯草杆菌][对地衣芽孢杆菌] Protease (Subtilisin A from Bacillus licheniformis) 货号：E-BSPRT-40ML Megazyme中文站

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蛋白酶[枯草杆菌][对地衣芽孢杆菌]

英文名：Protease (Subtilisin A from Bacillus licheniformis)

货号：E-BSPRT-40ML

规格：2 grams – 40ML

High purity Protease (Subtilisin A from B. licheniformis) (liquid) for use in research, biochemical enzyme assays an in vitro diagnostic analysis.

EC 3.4.21.14

Protease.

From Bacillus licheniformis. Highly purified. In 50% glycerol. For use in Megazyme Total Dietary Fiber test method. 

Specific activity: ~ 6 U/mg of protein; 300 U/ml (40oC, pH 8.0, casein as substrate). 

Stable at 4oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

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4-甲基β-laminaritetraoside 4-Methylumbelliferyl-β-laminaritetraoside 货号：O-4MULAM4 Megazyme中文站

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4-甲基β-laminaritetraoside

英文名：4-Methylumbelliferyl-β-laminaritetraoside

货号：O-4MULAM4

规格：5 mg

Synonym: MU-β-laminaritetraoside, 
                 4MU-β-laminaritetraoside
CAS: 618879-31-5
Molecular Formula: C34H48NO23
Molecular Weight: 824.7
Purity: > 98%

High purity 4-Methylumbelliferyl-β-laminaritetraoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a flourimetric substrate for the measurement/detection of 1,3-β-glucan degrading enzymes.

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纤维素酶[1，4-D-葡聚糖内切酶 [Emersonii篮状菌] Cellulase (endo-1，4-β-D-glucanase) (Talaromyces emersonii) 货号：E-CELTE Megazyme中文站

发表于由

纤维素酶[1，4-D-葡聚糖内切酶 [Emersonii篮状菌]

英文名：Cellulase (endo-1，4-β-D-glucanase) (Talaromyces emersonii)

货号：E-CELTE

规格：2000 Units

High purity Cellulase (endo-1,4-β-D-glucanase) (T. emersonii) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4 
CAZy Family: GH5

From Talaromyces emersonii. Two bands on SDS PAGE. 
In 3.2 M ammonium sulphate.

Specific activity: 64 U/mg (40oC, pH 4.5, CM-cellulose 4M as substrate).

Stable at 4oC for > 4 years.

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作用于高支链和不可溶解纤维质纤维素内切酶唯一检测底物 Cellazyme T – 1000 Tablets 货号：T-CTZ-1000T Megazyme中文站

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作用于高支链和不可溶解纤维质纤维素内切酶唯一检测底物

英文名：Cellazyme T – 1000 Tablets

货号：T-CTZ-1000T

规格：1000 Tablets

High purity dyed and crosslinked Cellazyme T tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. 

A unique substrate for the measurement of endo-cellulases active on highly branched and insoluble cellulosic substrates. Containing AZCL-Xyloglucan. Highly sensitive substrate.

作用于高支链和不可溶解纤维质纤维素内切酶唯一检测底物

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32α-L-阿糖呋喃-木二糖（A3X） 32-α-L-Arabinofuranosyl-xylobiose (A3X) 货号：O-A3X Megazyme中文站

发表于由

32α-L-阿糖呋喃-木二糖（A3X）

英文名：32-α-L-Arabinofuranosyl-xylobiose (A3X)

货号：O-A3X

规格：10 mg

CAS: 144938-89-6
Molecular Formula: C15H26O13
Molecular Weight: 414.4
Purity: > 95%

High purity 32-α-L-Arabinofuranosyl-xylobiose for or use in research, biochemical enzyme assays and in vitro diagnostic analysis. It can be used as an analytical standard or as a substrate to help characterise the activities of arabinoxylan degrading enzymes including endo-xylanase, β-xylosidase and α-L-arabinofuranosidase. This compound was prepared by the controlled enzymatic hydrolysis of wheat arabinoxylan. 

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阿魏酸酯酶[丁酸杆菌] Feruloyl esterase (Clostridium thermocellum) 货号：E-FAEZCT Megazyme中文站

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阿魏酸酯酶[丁酸杆菌]

英文名：Feruloyl esterase (Clostridium thermocellum)

货号：E-FAEZCT

规格：10 Units (~ 1800 U on FAXX)

High purity recombinant Feruloyl esterase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.1.1.73 
CAZy Family: CE1

Recombinant. Feruloyl esterase domain of xylanase XynZ (Xyn10A) from Clostridium thermocellum. 
In 3.2 M ammonium sulphate.

Specific activity: ~ 0.6 U/mg on ethyl ferulate at 50oC and pH 6.0. This enzyme is ~ 180 fold more active on FAXX.

Stability: > 2 years at 4oC.

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谷氨酸草酰乙酸转氨酶[大肠杆菌] Glutamate oxaloacetate transaminase (E. coli) 货号：E-GOTEC Megazyme中文站

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谷氨酸草酰乙酸转氨酶[大肠杆菌]

英文名：Glutamate oxaloacetate transaminase (E. coli)

货号：E-GOTEC

规格：5000

谷氨酸草酰乙酸转氨酶[大肠杆菌]

High purity Glutamate oxaloacetate transaminase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.6.1.1
From E. coli. In 3.2 M ammonium sulphate. Electrophoretically homogeneous.
Specific activity: ~ 40 U/mg (25°C, pH 8.5).
Stable at 4°C for > 4 years

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普鲁兰酶M1[植生克雷伯氏菌] Pullulanase M1 (Klebsiella planticola) 货号：E-PULKP Megazyme中文站

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普鲁兰酶M1[植生克雷伯氏菌]

英文名：Pullulanase M1 (Klebsiella planticola)

货号：E-PULKP

规格：700 Units

High purity Pullulanase M1 (K. planticola) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.41 
CAZy Family: GH13

From Klebsiella planticola. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.

Specific activity: 34 U/mg (40oC, pH 5.0, 1% pullulan). 

Stable at 4oC for > 4 years. 
Recommended pullulanase for research on starch structure.

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