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Lactate Dehydrogenase (LDH-P) 乳酸脱氢酶
- 临床检测用酶
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
| 由来 | porcine heart |
|---|---|
| 系统名称 |
(S )-Lactate : NAD+ oxidoreductase |
| EC编号 | 1.1.1.27 |
| 反应式 |
Pyruvate + NADH + H+ L-Lactate + NAD+ |
规格
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧150 U/mg | |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 115 kDa (gel filtration) |
|---|---|
| Michaelis constant | 2.5×10-2M (fructosyl-glycine) 1.0×10-4M (pyruvate) |
| pH Optimum | 7.4–8.0 |
| pH Stability | 7.0–7.5 |
| Optimum temperature | 50–55℃ |
| Thermal stability | below 45℃ |
适用类型
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
参考文献
- Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
- Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
- Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
- Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).
- 产品单页
- SDS