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Products > Crystallization Screens > Grid Screens > Grid Screen Ammonium Sulfate
Grid Screen Ammonium Sulfate
Applications
- Primary or secondary, Ammonium sulfate versus pH grid crystallization screen for biological macromolecules
Features
- A systematic grid screen varying Ammonium sulfate concentration versus pH
- Samples pH 4 to 9
- Samples four concentrations of Ammonium sulfate
- Precise pH versus Ammonium sulfate screen
- pH adjusted after salt addition; 25°Celsius
- Combine reagents between rows or columns to create expanded grid screens
Description
Grid Screen™ Ammonium Sulfate is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.
The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Ammonium sulfate between pH 4.0 and 9.0. Grid Screen Ammonium Sulfate systematically evaluates Ammonium sulfate at four concentrations (0.8, 1.6, 2.4, 3.0 M) versus 6 pH levels (4, 5, 6, 7, 8, 9).
Grid Screen Ammonium sulfate consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salt and buffers. Individual reagents are available through the Hampton Research Custom Shop.
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CAT NO
HR2-211
NAME
DESCRIPTION
10 ml, tube format
PRICE
$138.00
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Support Material(s)
HR2-211 Grid Screen Ammonium Sulfate Documents
HR2-211 Grid Screen Ammonium Sulfate SDS
Grid Screen Ammonium Sulfate Formulation & Scoring Data Related Item(S)
- Individual Grid Screen Ammonium Sulfate reagents
References
1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.
2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.
3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.
4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.
5. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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