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Products > Crystallization Screens > Grid Screens > Grid Screen PEG/LiCl
Grid Screen PEG/LiCl
Applications
- Primary or secondary, PEG & Lithium chloride versus pH grid crystallization screen for biological macromolecules
Features
- A systematic grid screen varying Polyethylene glycol 6,000 concentration in Lithium chloride versus pH
- Samples pH 4 to 9
- Samples four concentrations of Polyethylene glycol 6,000 in the presence of 1.0 M Lithium chloride
- Precise pH versus PEG and Lithium chloride screen
- pH adjusted after salt addition; 25°Celsius
- Combine reagents between rows or columns to create expanded grid screens
Description
Grid Screen™ PEG/LiCl is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.
The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in PEG and Lithium chloride between pH 4.0 and 9.0.
Grid Screen PEG/LiCl evaluates Polyethylene glycol 6,000 at four concentrations (0, 10, 20, 30 %w/v) in the presence of 1.0 M Lithium chloride versus six pH levels (4, 5, 6, 7, 8, 9).
Grid Screen PEG/LiCl consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity polymer, salt, and buffers. Individual reagents are available through the Hampton Research Custom Shop.
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CAT NO
HR2-217
NAME
DESCRIPTION
10 ml, tube format
PRICE
$138.00
cart quote
Support Material(s)
HR2-217 Grid Screen PEG/LiCl Documents
HR2-217 Grid Screen PEG/LiCl SDS
Grid Screen PEG/LiCl Formulation & Scoring Data Related Item(S)
- Individual Grid Screen PEG/LiCl Reagents
References
1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.
2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.
3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.
4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.
5. Expression, purification and preliminary structural analysis of the coiled-coil domain of Deinococcus radiodurans RecN. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1; 68(Pt 2): 218-221.
6. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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