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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-201
NDSB-201
Applications
- Crystallization grade NDSB-201 for formulating screens or for optimization
Features
- Efficiently solubilizes proteins at non-denaturing concentrations
- Useful in preventing protein aggregation
- Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
- Protein specific additive for solubility and stability
Description
The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.
Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.
Typical useful NDSB concentration in protein sample is 0.5-1.0 M.
NDSB-201
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C8H11NO3S
Mr 201.26
CAS Number [15471-17-7]
EC Number 239-491-3
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)
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CAT NO
HR2-701
NAME
DESCRIPTION
5 grams
PRICE
$43.00
cart quote
Support Material(s)
HR2-701 NDSB-201 User Guide
HR2-701 NDSB-201 SDS Certificate Of Analysis
References
1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).
2. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996).
3. Vuillard et al. J.Cryst Growth (1996) vol 168 pp 150-154.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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