密三糖或棉子糖/D-半乳糖检测试剂盒
英文名：Raffinose/D-Galactose Assay Kit
货号：K-RAFGA
规格：120 assays per kit
The Raffinose/D-Galactose test kit is specific and a rapid measurement and analysis of raffinose and D-galactose in plant materials and food products.
UV-method for the determination of Raffinose (also stachyose
and verbascose) and D-Galactose in legume seeds, plant
materials, foodstuffs and feed
Principle:
(α-galactosidase)
(1) Raffinose + stachyose + verbascose + H2O → D-galactose +
sucrose
(galactose mutarotase)
(2) α-D-Galactose ↔ β-D-galactose
(β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+
Kit size: 120 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 40 min
Detection limit: 5 mg/L
Application examples:
Cereal flours, soybean flour, by-products of sucrose manufacture
and other materials
Method recognition:
Used and accepted in food analysis
Advantages
- Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
Q1. Should the pH of the sample be adjusted even for samples in acidic media?
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
Q3. There is an issue with the performance of the kit; the results are not as expected.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
- Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
- Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
- State the kit lot number being used (this is found on the outside of the kit box).
- State which assay format was used (refer to the relevant page in the kit booklet if necessary).
- State exact details of any modifications to the standard procedure that is provided by Megazyme.
- State the sample type and describe the sample preparation steps if applicable.